Genetic Characterization of Three Distinct Mechanisms Supporting RNA-Driven DNA Repair and Modification Reveals Major Role of DNA Polymerase ?. In Western blotting, beta-actin (42 kDa) is commonly chosen as a loading control due to its broad and consistent expression across all eukaryotic cell types and the fact that expression levels of this protein are not affected by most experimental treatments (Figure 3). A pathogenic role for histone H3 copper reductase activity in a yeast model of Friedreich's ataxia. Bisphenol A exposure induces neurobehavioral deficits and neurodegeneration through induction of oxidative stress and activated caspase-3 expression in zebrafish brain. Therefore, proper sample preparation is critical. Under normoxic conditions, HIF-1 alpha has a short half-life and may be degraded within 5-8 minutes in both nuclear and cytoplasmic compartments. HIF-1 alpha is only stabilized at O2 concentrations below 5. It specifically labels -actin in a wide variety of tissues and species. Recognizes an epitope located on the N-terminal end of the -isoform of actin. This gene is located on human chromosome 7p22.1. Thioredoxin 1 (TRX1) Overexpression Cancels the Slow Force Response (SFR) Development. HIF-1 alpha can be a challenging target to work with in western blot. It is a cytoskeletal housekeeping protein. Long-Term Angiotensin II Infusion Induces Oxidative and Endoplasmic Reticulum Stress and Modulates Na+ Transporters Through the Nephron. Loading controls are essential for proper interpretation of western blots. Beta-actin, also known as a 'housekeeping' protein, is usually used as a loading control, for among others, the integrity of cells, protein degradation, in PCR and Western blotting. Publishing research using ab170325? Please let us know so that we can cite the reference in this datasheet.Īb170325 has been referenced in 14 publications. Beta-actin is a major constituent of the contractile apparatus.
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